recombinant human siglec (R&D Systems)
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
94
Structured Review
R&D Systems
recombinant human siglec
Recombinant Human Siglec, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+siglec/Recombinant+Human+Siglec-2%2FCD22+His-tag+Protein%2C+CF/pmc12964031-77-13-21
Average 94 stars, based on 2 article reviews
Recombinant Human Siglec, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+siglec/Recombinant+Human+Siglec-2%2FCD22+His-tag+Protein%2C+CF/pmc12964031-77-13-21
Average 94 stars, based on 2 article reviews
recombinant human siglec - by Bioz Stars,
2026-10
94/100 stars
Images
Related Articles
Recombinant:Article Title: Augmenting anti-CD19 and anti-CD22 CAR T-cell function using PD-1-CD28 checkpoint fusion proteins Article Snippet: On day 7-12 after transduction, cellular composition and transduction rate were analysed by flow cytometry using CD3-PE-Vio770, CD4-VioGreen, CD8-APC-Vio770, CD56-APC, c-myc-FITC (Miltenyi Biotec, Bergisch Gladbach, Germany) and PD-1-BV421 (Biolegend, San Diego, California, USA). .. To detect the anti-CD22 CAR, Article Title: A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells Article Snippet: .. The following antibodies, proteins, and dyes were used: PE- or BV421-conjugated anti-CD33 (clone WM53; BioLegend, San Diego, CA), PE-conjugated anti-mesothelin (clone REA1057; Miltenyi Biotec, Germany), PerCP-conjugated anti-CD19 (clone HIB19; BioLegend), PE- or FitC-conjugated anti-CD3 (clone SK7; BD Biosciences, Franklin Lakes, NJ), BV605-conjugated anti-CD4 (clone RPA-T4; BioLegend), PE-conjugate anti-CD34 to detect RQR8 (clone QBEND/10; Invitrogen, Carlsbad, CA), BV421-conjugated anti-PD-1 (clone EH12.2H7; BioLegend), PE-Cy7-conjugated anti-B2M (clone 2M2, BioLegend) BV650-conjugated anti-CD8 (clone RPA-T8; BioLegend), PE-Cy7-conjugated anti-IL-2 (clone MQ1-17H12; BioLegend), BV421-conjugated anti-IFNγ (clone 4S.B3; BioLegend), APC-conjugated anti-TNFɑ (clone Mab11; BioLegend), BV510-conjugated anti-CD107a (clone H4A3; BD Biosciences), Fixable viability dye eFluor 780 (eBioscience, Carlsbad, CA), Atto 647-conjugated recombinant human CD19 (BioTechne, Minneapolis, MN), Article Title: Digital polymerase chain reaction strategies for accurate and precise detection of vector copy number in chimeric antigen receptor T-cell products. Article Snippet: Background aims: Vector copy number (VCN), an average quantification of transgene copies unique to a chimeric antigen receptor (CAR) T-cell product, is a characteristic that must be reported prior to patient administration, as high VCN increases the risk of insertional mutagenesis.. Historically, VCN assessment in CAR T-cell products has been performed via quantitative polymerase chain reaction (qPCR). qPCR is reliable along a broad range of concentrations, but quantification requires use of a standard curve and precision is limited.. Digital PCR (dPCR) methods were developed for absolute quantification of target sequences by counting nucleic acid molecules encapsulated in discrete, volumetrically defined partitions. Article Title: Ten-year experience of CD22 CAR T cells for children and young adults with B-cell acute lymphoblastic leukemia Article Snippet: .. CD22 CAR detection was done using a commercially available fluorescently labeled recombinant protein: Article Title: Effect of Hypoxia on Siglec-7 and Siglec-9 Receptors and Sialoglycan Ligands and Impact of Their Targeting on NK Cell Cytotoxicity Article Snippet: .. Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Article Title: Self-associated molecular patterns mediate cancer immune evasion by engaging Siglecs on T cells Article Snippet: Tissue microarrays from US Biomax were used for screening of Siglec-9 ligand expression in lung cancer samples. .. For immunofluorescence staining, Recombinase Polymerase Amplification:Article Title: A Singular Base Editing Platform for Polyfunctional Multiplex Engineering of Immune Cells Article Snippet: .. The following antibodies, proteins, and dyes were used: PE- or BV421-conjugated anti-CD33 (clone WM53; BioLegend, San Diego, CA), PE-conjugated anti-mesothelin (clone REA1057; Miltenyi Biotec, Germany), PerCP-conjugated anti-CD19 (clone HIB19; BioLegend), PE- or FitC-conjugated anti-CD3 (clone SK7; BD Biosciences, Franklin Lakes, NJ), BV605-conjugated anti-CD4 (clone RPA-T4; BioLegend), PE-conjugate anti-CD34 to detect RQR8 (clone QBEND/10; Invitrogen, Carlsbad, CA), BV421-conjugated anti-PD-1 (clone EH12.2H7; BioLegend), PE-Cy7-conjugated anti-B2M (clone 2M2, BioLegend) BV650-conjugated anti-CD8 (clone RPA-T8; BioLegend), PE-Cy7-conjugated anti-IL-2 (clone MQ1-17H12; BioLegend), BV421-conjugated anti-IFNγ (clone 4S.B3; BioLegend), APC-conjugated anti-TNFɑ (clone Mab11; BioLegend), BV510-conjugated anti-CD107a (clone H4A3; BD Biosciences), Fixable viability dye eFluor 780 (eBioscience, Carlsbad, CA), Atto 647-conjugated recombinant human CD19 (BioTechne, Minneapolis, MN), Staining:Article Title: Digital polymerase chain reaction strategies for accurate and precise detection of vector copy number in chimeric antigen receptor T-cell products. Article Snippet: Background aims: Vector copy number (VCN), an average quantification of transgene copies unique to a chimeric antigen receptor (CAR) T-cell product, is a characteristic that must be reported prior to patient administration, as high VCN increases the risk of insertional mutagenesis.. Historically, VCN assessment in CAR T-cell products has been performed via quantitative polymerase chain reaction (qPCR). qPCR is reliable along a broad range of concentrations, but quantification requires use of a standard curve and precision is limited.. Digital PCR (dPCR) methods were developed for absolute quantification of target sequences by counting nucleic acid molecules encapsulated in discrete, volumetrically defined partitions. Article Title: Self-associated molecular patterns mediate cancer immune evasion by engaging Siglecs on T cells Article Snippet: Tissue microarrays from US Biomax were used for screening of Siglec-9 ligand expression in lung cancer samples. .. For immunofluorescence staining, Labeling:Article Title: Ten-year experience of CD22 CAR T cells for children and young adults with B-cell acute lymphoblastic leukemia Article Snippet: .. CD22 CAR detection was done using a commercially available fluorescently labeled recombinant protein: Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 CRISPR:Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Antibody Labeling:Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Software:Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Article Title: Optimization and validation of CAR transduction into human primary NK cells using CRISPR and AAV. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER 209Bi_pHH3 Fluidigm N/A 89Y_CD235 Fluidigm N/A Bacterial and virus strains AAV6 Andelyn Biosciences https://andelynbio.com Biological samples Buffy coat RedCross N/A Chemicals, peptides, and recombinant proteins Human recombinant IL-2 Protein STEMCELL Technologies Cat#15065 RosetteSepTM Human NK Cell Enrichment Cocktail STEMCELL Technologies Cat# 15065 Ficoll-Paque PLUS Cytiva Cat# 17144003 AIM-V Gibco Cat# 12055083 CTSTM Immune Cell SR Gibco Cat# A2596101 IDTE pH 7.5 (1X TE Solution) Integrated DNA Technologies, Inc. Cat# 11-01-02-02 Alt-R Cas9 Electroporation Enhancer, 10 nmol Integrated DNA Technologies Cat# 1075916 Alt-R S.p. .. HiFi Cas9 Nuclease V3, 500 mg Integrated DNA Technologies Cat# 1081061 Immunofluorescence:Article Title: Self-associated molecular patterns mediate cancer immune evasion by engaging Siglecs on T cells Article Snippet: Tissue microarrays from US Biomax were used for screening of Siglec-9 ligand expression in lung cancer samples. .. For immunofluorescence staining, |